ASTM E1280-1997(2008) 哺乳动物细胞诱变性用老鼠淋巴瘤鉴定实施的标准指南

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【英文标准名称】:StandardGuideforPerformingtheMouseLymphomaAssayforMammalianCellMutagenicity
【原文标准名称】:哺乳动物细胞诱变性用老鼠淋巴瘤鉴定实施的标准指南
【标准号】:ASTME1280-1997(2008)
【标准状态】:现行
【国别】:美国
【发布日期】:1997
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:F04.16
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:
【英文主题词】:Assay;Carcinogenicity;Chromosomal/geneticeffects;Cloning;Cytotoxicity;Dataanalysis--environmental;Geneticexamination;Mammaliancellmutagenicity;Metabolicactivation;Mice;MLA(mouselymphomaassay);Mutagenicity;Plating(cloning)efficiency;
【摘要】:Thisguideislimitedtoproceduresusedsolelyforthetestingofsubstancestodeterminetheirmutagenicityanddoesnotapplytoothermethodsandusessuchasexploringmechanismsofmutation.Recentevidencesuggeststhatthisassaymeasuresadualgeneticendpoint;therefore,somediscussionoftherelationshipsbetweenmammaliancellmutagenicitytestingresultsandtheresultsobservedbothinpuregenemutationalassaysandincytogeneticassaysisnecessary.However,itisnottheintentofthisguidetodiscussotherrelationshipsbetweenthismammaliancellmutagenicitytestingresultsandtheresultsobservedinothertestsformutagenicityandcarcinogenicity.1.1ThepurposeandscopeofthisguideistopresentbackgroundmaterialandtoestablishcriteriabywhichprotocolsandproceduresforconductingtheL5178Y/TK+/x2212;-3.7.2Cmouselymphomamutagenicityassay(commonlyreferredtoasthemouselymphomaassay,(MLA))canbeproperlyunderstoodandevaluated.Thisguideisalsointendedtoaidresearchersandotherstogainabetterunderstandingofthecriticalelementsinvolvedwithmammaliancellmutagenicitytesting.Morespecifically,thisguideisintendedtoprovideforresearcherstheaccomplishmentofthefollowinggoals:1.1.1Provideanunderstandingofthecriticalprocedures(steps)intheperformanceofthismammaliancellmutagenicitytest.1.1.2Providegeneralizedcriteriabywhichresearcherscanevaluateiftheyareproperlyperforming,utilizing,andinterpretingthisassay.1.1.3ProvidecriteriabywhichindividualsresponsibleforevaluatingMLAdatacandetermineiftheexperimentshavebeenproperlyperformedandinterpreted.1.1.4Provideabasisfromwhichnewproceduresanddevelopmentsintestingprocedurescanbeevaluated.1.1.5Provideanunderstandingofthetypesofgeneticdamage(thatis,geneandchromosomemutation)thatmaybedetectedinthismammaliancellmutagenicitytest.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.
【中国标准分类号】:B41
【国际标准分类号】:07_100_10
【页数】:9P.;A4
【正文语种】:英语